Respiratory Models
Lung Inflammation is a critical component of many lung diseases including Asthma. Asthma is classified as a chronic inflammatory disease of the airways, however the underlying physiology and immunological pathways are not completely understood. Allergen challenge models are commonly used to study mechanisms involved in lung inflammation and allergic Asthma. MLM Medical Labs offers validated and customizable models to study the inflammatory components involved in lung inflammation and Asthma.
LPS-induced Lung Inflammation/Acute Lung Injury (ALI) Screen
LPS lung inflammation models are widely used to study host-response patterns in the pulmonary compartment. The LPS lung inflammation model is an acute lung inflammation model where the pulmonary response following bacterial exposure can be observed. Using this model, it is possible to measure and characterize the cellular profile of recruited leukocytes to the lung airways as well as the levels of pro-inflammatory cytokines.
Available Readouts:
- Cell populations in the bronchoalveolar lavage fluid (BALF) by flow cytometry
- Cytokine profile in the BALF or lung tissue by multiplex
- White blood count
- Total protein
Ovalbumin (OVA) induced Allergic Asthma Model
Ovalbumin (OVA) induced Allergic Asthma Model
While we know many factors and events that play an important role in the initiation, progression and persistence of allergic Asthma, there is still a lot to be understood about the immuno-regulatory mechanisms. The OVA-induced Asthma model is a widely used model that results in the characteristic features of Asthma allowing the study and assessment of novel treatments.
Available Readouts:
- Cell populations in the bronchoalveolar lavage fluid (BALF) by flow cytometry
- Cytokine profile in the BALF or lung tissue by multiplex
- White blood count
- IgE levels
House Dust Mite (HDM) Induced Asthma Model
The House Dust Mite (HDM) model of allergic Asthma is used to assess therapies with potential indications in airway inflammation and/or pulmonary inflammation driven primarily by repeated exposure to allergens. As roughly 85% of allergic Asthma sufferers have increased HDM-specific serum IgE levels, the HDM model demonstrates significant relevance for investigating the molecular mechanisms of allergic Asthma pathogenesis.
HDM-induced Asthma recapitulates many of the features of human allergic Asthma, including a primarily Th2- associated cytokine response, eosinophilic infiltration into the lungs, airway hyper responsiveness (AHR) and epithelial tissue remodeling.
Available readouts:
- Cell populations in the bronchoalveolar lavage fluid (BALF) by flow cytometry
- Cytokine profile in the BALF or lung tissue by multiplex
- White blood count
- IgE levels
- Total protein
Bleomycin-induced Fibrosis (IPF)
Bleomycin has been used to induce lung injury to find efficacious treatments for pulmonary fibrosis for over a decade. At MLM Medical Labs, we have refined the oral aspiration administration to allow for more even distribution of disease throughout both right and left lungs, making bleomycin-induced injury a reliable murine model for preclinical research. This is a great model for studying idiopathic pulmonary fibrosis (IPF).
Available readouts options:
- Immunophenotyping of bronchoalveolar lavage fluid (BALF):
- Quantitation of T Cells, B Cells, Macrophage, Neutrophils, Eosinophils, Fibroblasts, etc.
- Cytokine Expression Levels (ELISA/Multiplex) in:
- BALF
- Lung Tissue Homogenate
- Plasma/Serum
- Histology of lung:
- H&E for morphologic analysis, cellular infiltration, etc.
- Masson’s Trichrome staining for collagen
- IHC for specific markers of interest such as CD3, CD11b, etc.
- Lung tissue biomarkers
- Blood biomarkers (protein or nucleic acid)
- Lung tissue cellularity
Are you interested in a variation of a model or a new paradigm?
Our scientists are passionate about the development of new models! We often participate in opportunities for co-development and pilot programs to establish new models and reach new levels of understanding.