Cell Culture Modeling
Evaluate the Immunosuppression or Immunogenicity Properties of Compounds
MLM Medical Labs offers a variety of assays for evaluating select pathways. ImmuneProfiler is a suite of assays that can be customized to determine a compound’s properties on the immune response. Our customers can select pathways of interest, choose between stimulation or inhibition assays, and evaluate in cell-based systems.
In Vitro Immune Response Assays
|
Pathway
|
System
|
Stimulant
|
Output
|
|---|---|---|---|
|
Th17 activation
|
PBMC
|
antiCD3
|
cytokine panel, FACS
|
|
T cell inhibition
|
PBMC
|
antiCD3/CD28, ConA, PHA
|
cytokine panel, FACS
|
|
T cell stimulation
|
PBMC
|
sponsor compound
|
cytokine panel, FACS
|
|
Cytokine Release
|
PBMC
|
sponsor compound
|
cytokine panel, FACS
|
|
Macrophage (Inhibition)
|
PBMC
|
LPS
|
cytokine panel, FACS
|
|
Macrophage (stimulation)
|
PBMC
|
sponsor compound
|
cytokine panel, FACS
|
|
Proliferation - (stimulate)
|
PBMC
|
sponsor compound
|
CFSE
|
|
Proliferation - (inhibition)
|
PBMC
|
anti-CD3/CD28, ConA, PHA
|
CFSE
|
Readouts include:
- Specific cytokine panels, FACs analyses
- Th1 Th2, Th17, CD8, B-cell response in vivo
- Macrophage and T-cell inhibition and stimulation, proliferation and cytokine release in vitro