Cell Culture Modeling

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Evaluate the Immunosuppression or Immunogenicity Properties of Compounds

MLM Medical Labs offers a variety of assays for evaluating select pathways. ImmuneProfiler is a suite of assays that can be customized to determine a compound’s properties on the immune response. Our customers can select pathways of interest, choose between stimulation or inhibition assays, and evaluate in cell-based systems.

In Vitro Immune Response Assays

Pathway
System
Stimulant
Output
Th17 activation
PBMC
antiCD3
cytokine panel, FACS
T cell inhibition
PBMC
antiCD3/CD28, ConA, PHA
cytokine panel, FACS
T cell stimulation
PBMC
sponsor compound
cytokine panel, FACS
Cytokine Release
PBMC
sponsor compound
cytokine panel, FACS
Macrophage (Inhibition)
PBMC
LPS
cytokine panel, FACS
Macrophage (stimulation)
PBMC
sponsor compound
cytokine panel, FACS
Proliferation - (stimulate)
PBMC
sponsor compound
CFSE
Proliferation - (inhibition)
PBMC
anti-CD3/CD28, ConA, PHA
CFSE

Readouts include:

  • Specific cytokine panels, FACs analyses
  • Th1 Th2, Th17, CD8, B-cell response in vivo
  • Macrophage and T-cell inhibition and stimulation, proliferation and cytokine release in vitro