Pharmacology & Efficacy Studies for Lung Inflammation & Asthma
Lung Inflammation is a critical component of many lung diseases including asthma. Asthma is classified as a chronic inflammatory disease of the airways, however the underlying physiology and immunological pathways are not completely understood. Allergan challenge models are commonly used to study mechanisms involved in lung inflammation and allergic asthma. Ovalbumin-induced allergic asthma is a widely used model to reproduce the airway eosinophilia, pulmonary inflammation and elevated IgE levels found during asthma.
MLM Medical Labs offers models to study the inflammatory components involved in lung inflammation and asthma.
Don't see a readout or model listed below? Our scientists are experienced in validating models quickly from the literature.
Contact a scientist to discuss your model requirements.
Lung Injury Models
LPS-Induced Lung Inflammation/Acute Lung Injury (ALI) Screen
Ovalbumin (OVA) induced Allergic Asthma Efficacy Model
Commonly Utilized Model of Airway Inflammation
House Dust Mite (HDM) induced Asthma
Allergic Asthma Model with High Translational Value
The House Dust Mite (HDM) murine model of allergic asthma is used to assess therapies with potential indications in airway inflammation and/or pulmonary inflammation driven primarily by repeated exposure to allergens. As roughly 85% of allergic asthma sufferers have increased HDM-specific serum IgE levels, the HDM model demonstrates significant relevance for investigating the molecular mechanisms of allergic asthma pathogenesis.
HDM-induced asthma recapitulates many of the features of human allergic asthma, including a primarily Th2- associated cytokine response, eosinophilic infiltration into the lungs, airway hyper responsiveness (AHR) and epithelial tissue remodeling. The model consists of a subcutaneous HDM sensitization followed by intranasal challenge of HDM. Main readouts include broncheoalveolar lavage fluid (BALF) cellularity assessment and cytokine production, with optional blood and lung tissue collection for further downstream analysis.
- Cell populations in the BALF by FACS
- Cytokine profile in the BALF or lung tissue homogenate by multiplex
- IgE levels
- Total Protein