The use of flow cytometry has been established already back in 2017 at MLM, but due to the increasing use for safety and/or exploratory purposes in clinical trials an advanced flow cytometer was installed recently. Based on our previous experience with the FC500 from Beckman Coulter we have chosen the CytoFLEX V2-B5-R3 from the same manufacturer. This device has 3 active lasers at 405nm, 488nm and 638nm and ten channels for fluorescence detection and can be upgraded with additional red, blue and violet channels.
The flow cytometer is a very versatile lab device and its application areas in clinical trials are numerous. Size, activation state or the expression of biomarkers of specific cell populations can quantified, only to mention some examples. We have currently established panels to quantify lymphocytes with the following surface markers: CD3, CD4, CD8 and CD27 on T cells, CD19 and CD20 on B-lymphocytes. Amongst others these markers are used to characterize Non-Hodgkin or Hodgkin lymphoma. To identify B-Cell-lymphomas, changes in the expression of immunoglobulins IgM and IgD can be analyzed. The set of markers can be expanded and customized panels have been implemented for other studies.
The CytoFLEX definitely expands MLM’s lab expertise in the field of oncology and immunology. To find out more about our specialty lab parameters, eg. cytokines, growth factors and tumor markers, please refer to our list of parameters.
If you are missing parameters of interest to you, please don’t hesitate to contact Dr. Katja Neuer, Director Business Development, kneuer(at)mlm-labs(dot)com. There is a good chance that we have validated your parameter of interest in the meanwhile, since the list is constantly growing.